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Peng Guo, Yi-Ping Qi, Lin-Tong Yang, Ning-Wei Lai, Xin Ye, Yi Yang, Li-Song Chen*.Root Adaptive Responses to Aluminum-Treatment Revealed by RNA-Seq in Two Citrus Species With Different Aluminum-Tolerance


发布者:曹海雷    发布时间:2017-03-09    浏览次数:186

 

所有作者:Peng Guo, Yi-Ping Qi, Lin-Tong Yang, Ning-Wei Lai, Xin Ye, Yi Yang, Li-Song Chen*

论文题目:Root Adaptive Responses to Aluminum-Treatment Revealed by RNA-Seq in Two Citrus Species With Different Aluminum-Tolerance

来源期刊:Frontiers in Plant Science

发表日期:08 March 2017

原文链接:

http://journal.frontiersin.org/article/10.3389/fpls.2017.00330/full

  

Abstract:

Seedlings of aluminum (Al)-tolerantCitrus sinensisand Al-intolerantCitrus grandiswere fertigated daily with nutrient solution containing 0 and 1.0 mM AlCl3●6H2O for 18 weeks. The Al-induced decreases of biomass and root total soluble proteins only occurred inC. grandis, demonstrating thatC. sinensishad higher Al-tolerance thanC. grandis. Under Al-treatment,C. sinensisroots secreted more citrate and malate thanC. grandisones; less Al was accumulated inC. sinenisthan inC. grandisleaves. The Al-induced reduction of phosphorus was lesser inC. sinensisroots and leaves than inC. grandisones, whereas the Al-induced increase of sulfur was greater inC. sinensisroots and leaves. Using RNA-seq, we isolated 1905 and 2670 differentially expressed genes (DEGs) from Al-treatedC. sinensisthanC. grandisroots, respectively. Among these DEGs, only 649 DEGs were shared by the two species. Further analysis suggested that the following several aspects conferredC. sinensishigher Al-tolerance: (a) Al-treatedC. sinensisseedlings had a higher external Al detoxification capacityviaenhanced Al-induced secretion of organic acid anions, a higher antioxidant capacity and a more efficient chelation system in roots; (b) Al-treatedC. sinensisseedlings displayed a higher level of sulfur in roots and leaves possibly due to increased uptake and decreased export of sulfur and a higher capacity to maintain the cellular phosphorus homeostasis by enhancing phosphorus acquisition and utilization; (c) Cell wall and cytoskeleton metabolism, energy and carbohydrate metabolism and signal transduction displayed higher adaptative responses to Al inC. sinensisthan inC. grandisroots; (d) More upregulated than downregulated genes related to fatty acid and amino acid metabolisms were isolated from Al-treatedC. sinensisroots, but the reverse was the case for Al-treatedC. grandisroots. These results provide a platform for further investigating the roles of genes possibly responsible for citrus Al-tolerance.

  

  

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